microspheres  (Yeasen Biotechnology)

Journal: Bioactive Materials

Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential

doi: 10.1016/j.bioactmat.2026.01.033

Figure Lengend Snippet: Schematic illustration of injectable hydrogel microspheres derived from the skin secretions of Andrias davidianus exhibit unique wet-adhesion properties mediated by multiple interaction forces, enabling robust tissue adhesion in dynamic physiological environments. These bioadhesive microspheres serve as versatile platforms for localized delivery of therapeutic cells and pharmaceutical agents. Preclinical studies demonstrated their significant regenerative potential across multiple tissue repair models, including periodontitis-induced bone loss, articular cartilage defects, and chronic diabetic wounds.

Article Snippet: In order to observe the morphology and cytoskeleton of the cells adhered to the microspheres, FITC-phalloidin (Yeasen, China) was used to stain the cartilage stem cells loading on the microspheres after 24 h of culture.

Techniques: Derivative Assay

Journal: Bioactive Materials

Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential

doi: 10.1016/j.bioactmat.2026.01.033

Figure Lengend Snippet: Fabrication and characterization of SGM. a) Schematic illustration of the preparation workflow for SGM. b ) Microscopic image of spherical morphology of microspheres. c ) SEM micrographs revealing the porous architecture of lyophilized microsphere. d ) Quantitative size distribution analysis of microspheres. e ) FTIR spectral analysis confirming successful conjugation while preserving characteristic functional groups of GelMA and SSAD. f ) Surface charge characterization via zeta potential measurement. g ) Proposed adhesion mechanism involving synergistic interfacial interactions (hydrogen bonding, hydrophobic effects, van der Waals forces and cation-π interactions). h ) Comparative drug release kinetics showing sustained delivery profiles of microspheres. i ) The adhesion of GM and SGM to agarose and subsequent drug release. Fluorescence microscope demonstrating the tissue permeability of drug-loaded microspheres.

Article Snippet: In order to observe the morphology and cytoskeleton of the cells adhered to the microspheres, FITC-phalloidin (Yeasen, China) was used to stain the cartilage stem cells loading on the microspheres after 24 h of culture.

Techniques: Conjugation Assay, Preserving, Functional Assay, Zeta Potential Analyzer, Fluorescence, Microscopy, Permeability

Journal: Bioactive Materials

Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential

doi: 10.1016/j.bioactmat.2026.01.033

Figure Lengend Snippet: Functional characterization of cell-laden microspheres. a ) Schematic illustration of cell-laden microspheres fabrication. b ) Longitudinal viability assessment by Live/dead cell staining (green: live cells; red: dead cells) at day 0, 3 and 7 post-encapsulation. c ) Live/Dead cells percentage (%). d ) F-actin Cytoskeletal staining (red) and nuclear morphology (blue) with corresponding semi-quantitative analysis. The cytoskeleton was stained in red and the nuclei in blue. e ) Mean fluorescence intensity of cytoskeletal staining. f ) Time-dependent migratory capacity of encapsulated cells evaluated at day 3, 7 and 14. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In order to observe the morphology and cytoskeleton of the cells adhered to the microspheres, FITC-phalloidin (Yeasen, China) was used to stain the cartilage stem cells loading on the microspheres after 24 h of culture.

Techniques: Functional Assay, Staining, Encapsulation, Fluorescence

Journal: Bioactive Materials

Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential

doi: 10.1016/j.bioactmat.2026.01.033

Figure Lengend Snippet: Wet adhesiveness of SGM in simulated physiological environments. a ) Schematic of hydrodynamic challenge simulating saliva or gingival crevicular fluid over microspheres on alveolar bone (Created with BioRender.com ). b ) Clinical periodontitis image (human) and reconstructed periodontal defect in the pig's alveolar bone. c ) SGM retention on the pig's cartilage under constant-flow irrigation. d ) Quantitative analysis of microsphere retention. e ) Schematic of hydrodynamic challenge simulating synovial fluid flow over microspheres on cartilage (Created with BioRender.com ). f ) Clinical osteoarthritis image (human) and reconstructed equivalent osteochondral defect in the pig's knee joint. g ) SGM retention on the pig's cartilage under constant-flow irrigation. h ) Quantitative analysis of microsphere retention. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In order to observe the morphology and cytoskeleton of the cells adhered to the microspheres, FITC-phalloidin (Yeasen, China) was used to stain the cartilage stem cells loading on the microspheres after 24 h of culture.

Techniques:

Journal: Bioactive Materials

Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification

doi: 10.1016/j.bioactmat.2026.02.050

Figure Lengend Snippet: Preparation of the D-Bmp2@M system and its therapeutic mechanism for osteoporosis fractures. Bmp2 fused with DSS6 were expressed in HEK293T and then encapsulated in porous PLGA microspheres to construct the D-Bmp2@M system. Upon injection into the osteoporotic fracture site, the system gradually releases D-Bmp2 as the microspheres degrade over approximately 30 days. The released D-Bmp2 actively binds to bone tissue due to the affinity of DSS6 for bone. This localized enrichment promotes osteogenic activity at the fracture site, promoting fracture healing while reducing the risk of ectopic bone formation. The sustained-release and targeted delivery systems provides a superior therapeutic strategy for fracture treatment.

Article Snippet: We observed the healing properties of the microsphere surface using a JSM-6700F scanning electron microscope (SEM, JEOL, Japan).

Techniques: Construct, Injection, Activity Assay

Journal: Bioactive Materials

Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification

doi: 10.1016/j.bioactmat.2026.02.050

Figure Lengend Snippet: Preparation and characterization of self-healing sustained-release microspheres loaded with D-Bmp2. a. Representative SEM images of microspheres before (top) and after (bottom) healing; left scale bar: 10 μm; middle and right scale bar: 2.5 μm. b. Statistical analysis of the microsphere diameter before and after healing determined via SEM. c. Representative confocal microscopy images of protein-loaded microspheres: PLGA microspheres (red) and Cy5-labeled D-Bmp2 (blue). Scale bar: 2 μm. d. Morphology of lyophilized D-Bmp2@M powder. e. SDS-PAGE of lyophilized D-Bmp2@M powder at different storage times. f. Representative firefly luciferase images from bioactivity assays of lyophilized D-Bmp2@M powder at different times. g. Activity change curve of lyophilized D-Bmp2@M powder at different time points (n = 3 per group). h, i. In vitro fluorescence intensity changes of Cy7-labeled D-Bmp2 from microspheres: (h) Representative fluorescence images of Cy7-D-Bmp2 maintained in microspheres (0–30 days) (top) and representative SEM images of microsphere degradation at different time points. Scale bar: 2.5 μm (bottom); (i) Relative fluorescence intensity change of Cy7-D-Bmp2 maintained in microspheres (n = 3 per group). j. Representative firefly luciferase images from Bmp2 reporter assays. k. Protein activity normalization: ratio of luminescence intensity (data from ) to protein concentration (data from ) (n = 3 per group). The data are presented as the means ± SDs. One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant).

Article Snippet: We observed the healing properties of the microsphere surface using a JSM-6700F scanning electron microscope (SEM, JEOL, Japan).

Techniques: Confocal Microscopy, Labeling, SDS Page, Luciferase, Activity Assay, In Vitro, Fluorescence, Protein Concentration

Journal: Bioactive Materials

Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification

doi: 10.1016/j.bioactmat.2026.02.050

Figure Lengend Snippet: In vitro validation of D-Bmp2@M osteogenic efficacy and inhibition of ectopic ossification. a. Schematic diagram of the osteoblast-bone Transwell model. Bmp2/D-Bmp2@M microspheres or free Bmp2/D-Bmp2 were loaded in the upper chambers, MC3T3-E1 cells were cultured on two coverslips (one of which was precoated with HA) in the lower compartments, and the medium was refreshed every day for 7 or 14 days. Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining were performed at days 7 and 14, respectively. b. Osteogenic differentiation staining: ALP (early-stage, day 7) and ARS (late-stage, day 14) staining. Scale bar: 200 μm. c. ALP activity was quantitatively analyzed using an ALP kit (n = 3 per group). d. Relative quantitative analysis of ARS staining was performed at an OD of 562 nm (n = 3 per group). e. qPCR analysis of Bmp2 signaling-related mRNA in MC3T3-E1 cells (n = 3 per group). f. Schematic diagram of the muscle-bone Transwell model. Bovine bone slices were co-incubated with Bmp2/D-Bmp2@M or free Bmp2/D-Bmp2 in the upper chambers, and C2C12 cells were cultured in the lower chambers and the medium was refreshed every day for 7 days. D-Bmp2 and Bmp2 retention on bone slices and ALP staining of C2C12 cells were analyzed on day 7. g. Representative fluorescence imaging of bone slices incubated with AF647-conjugated anti-Flag antibodies (above) (yellow arrows: bone slice) and C2C12 ALP staining images (below), scale bar: 200 μm. h. AF647-conjugated anti-Flag antibody fluorescence intensity quantification in bone slices (n = 3 per group). i. Quantification of ALP activity in C2C12 cells (n = 3 per group). j. qPCR analysis of Bmp2 signaling-related mRNA in C2C12 cells (n = 3 per group). The data are presented as the means ± SDs. One-way ANOVA was used for multiple comparisons. Significance levels: ns (not significant), ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: We observed the healing properties of the microsphere surface using a JSM-6700F scanning electron microscope (SEM, JEOL, Japan).

Techniques: In Vitro, Biomarker Discovery, Inhibition, Cell Culture, Staining, Activity Assay, Incubation, Fluorescence, Imaging

Journal: Bioactive Materials

Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification

doi: 10.1016/j.bioactmat.2026.02.050

Figure Lengend Snippet: In vivo testing of release kinetics and bone accumulation of D-Bmp2@M. a. Representative fluorescence images showing the changes in Cy7 fluorescence after local injection. b. Quantitative analysis of the changes in relative fluorescence intensity (n = 6 per group). c. Representative ex vivo fluorescence images of bone tissues at 1 day post-injection of free Cy7-D-Bmp2 or Cy7-Bmp2, along with quantitative analysis of the bone fluorescence intensity (n = 6 per group). d. Representative IFHC images at 1 day post-injection showing Bmp2 or D-Bmp2 localization and the bone to muscle fluorescence intensity ratio (n = 6 per group). IFHC: anti-Flag antibody (yellow), DAPI (blue); the white dotted line represents the boundary between bones and muscles (M: muscle, B: bone); scale bar: 20 μm. e. Representative ex vivo fluorescence images of bone tissues at 6 days post-injection of PLGA microspheres loaded with Cy7-D-Bmp2 or Cy7-Bmp2, along with quantitative analysis of the bone fluorescence intensity (n = 6 per group). f. Representative IFHC images at 6 days post-injection showing Bmp2 or D-Bmp2 localization and the bone-to-muscle fluorescence intensity ratio (the fluorescence intensity of the 10-μm bone boundary to muscle tissue) (n = 6 per group). IFHC: anti-Flag antibody (yellow), DAPI (blue); the white arrows highlight PLGA microspheres; the white dotted line represents the boundary between bones and muscles (M: muscle, B: bone); scale bar: 20 μm. The data are presented as the means ± standard deviations (SDs). Unpaired Student's t -test was used for two-group comparisons. Significance levels: ∗∗∗∗ p < 0.0001.

Article Snippet: We observed the healing properties of the microsphere surface using a JSM-6700F scanning electron microscope (SEM, JEOL, Japan).

Techniques: In Vivo, Fluorescence, Injection, Ex Vivo, Muscles